| - | Flight A |
| 16 | Jay Xiao (JHUW) def. Zoe Klapman (MU) 4-6, 7-5, 1-0 (12-10) |
| QF | Casey Accola (USNA) def. MaryBeth Hurley (JHUW) 6-2, 4-6, 1-0 (10-6) |
| QF | Stella Ribaudo (USNA) def. Jay Xiao (JHUW) 6-2, 4-6, 1-0 (10-8) |
| - | Flight B |
| 16 | Maitree Root (GMU) def. Ali Bader (JHUW) 6-4, 6-0 |
| 16 | Kamila Wong (JHUW) def. Rebecca Stepleman (UMW) 6-1, 6-1 |
| QF | Kamila Wong (JHUW) def. Avery Hopkey (LU) 2-6, 6-1, 1-0 (10-8) |
| SF | Syliva Eklund (USNA) def. Kamila Wong (JHUW) 7-6 (7-2), 6-1 |
| - | Flight C |
| 16 | Essence Wang (JHUW) def. Jordyn Pax (MU) 5-5, retired |
| 16 | Isabella Venter (SBU) def. Niamh Harding (JHUW) 6-2, 6-0 |
| QF | Parvathi Shanker (USNA) def. Essence Wang (JHUW) 6-4, 6-2 |
| - | Flight D |
| 16 | Celina Liu (JHUW) def. Abby Ackroyd (BU) 6-1, 2-6, 1-0 (11-9) |
| QF | Imani Ghosh (JHUW) def. Valeria Gonzalez (BU) 6-3, 6-3 |
| QF | Celina Liu (JHUW) def. Riley Smith (UMW) 6-3, 6-2 |
| SF | Imani Ghosh (JHUW) def. Christina Danford (USNA) 6-4, 6-2 |
| SF | Kashish Bote (SBU) def. Celina Liu (JHUW) 6-2, 6-2 |
| FI | Imani Ghosh (JHUW) def. Kashish Bote (SBU) 6-4, 6-3 |
| - | Flight E |
| 16 | Emily Javedan (JHUW) def. Brielle DeRoberts (BU) 6-0, 6-0 |
| 16 | Zoe Hsieh (JHUW) def. Madison McCarty (UMW) 6-2, 6-2 |
| QF | Hanna Elks Smith (USNA) def. Emily Javedan (JHUW) 7-6 (7-3), 7-5 |
| QF | Zoe Hsieh (JHUW) def. Bella Peters (LU) 6-0, 7-6 (7-1) |
| SF | Hanna Elks Smith (USNA) def. Zoe Hsieh (JHUW) 6-1, 6-3 |
